P-58: BSO-Induced Oxidative Stress Affects Testicular Ultrastructure, Levels of Oxidative Markers, Testosterone and Sperm Fertility

Background: GSH is the main intracellular antioxidant and its level is important for protecting cell from oxidative stress. BSO inhibits GSH synthesis and cause oxidative stress. The study is designed to investigate the effect of BSO induced oxidative stress on testicular histology, semen parameters and sperm fertility. Materials and Methods: The adult male mice were divided into two groups of control and experiment. In the experimental group the mice received 2 mmol /kg BSO for 35 days as IP injection. All mice were sacrificed at the end of the study and their testes were prepared for electron microscopy. For determination of SOD, MDA, GPX, GSH and testosterone levels, blood samples were obtained from heart. For evaluation of fertility the oocytes were obtained from superovulated mice by tubal flashing and inseminated with sperms collected from cauda epididymis. Embryo formation was considered as fertility success and compared in between groups. Results: Electron microscopy showed that in experimental group, germ cells were detached from basement membrane and were separated from each other and sertoli cells by large vacuoles. Determination of oxidative markers and testosterone level revealed that the concentration of SOD, GPX, GSH and testosterone in experimental group, in comparison with control group, is reduced while the level of MDA was increased (p<0.05). Sperm fertility, based on embryo formation, was decreased in experimental group. Conclusion: Ultrastractural changes of mice testes in Bso-induced oxidative stress is accompanied by decreasing of antioxidants and fertility rate.